FastQCFastQC Report
Mon 27 Feb 2023
SRR3127544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392456
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221960.9277495594485332No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG47630.19908412108728435No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC45530.19030653019324076No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG45050.18830022370317365No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39350.1644753341336267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72800.083.014841
TAGCGGG10250.074.9390262
CGTAGGG6650.074.4066852
ACGGGTA1400.074.052364
AGGGATG34650.072.288385
TAGGGCA15250.071.381624
GAATAGG15950.070.046541
TAAGGGA19650.070.026853
AGGGCAT24000.069.903925
AAGGGTA19450.068.566374
AAGAGGG82050.068.28852
ATAACGG2900.068.273221
GGTAAGG13200.067.854511
TAGAGGG44250.067.62462
AGTAAGG14800.067.526341
ATAGCGG4350.067.1895141
ATAGGGA23100.067.116333
CGAAGGG22050.067.106612
AGGGAAT26000.066.876415
AGGGCAC21100.066.8164445
Produced by FastQC (version 0.11.5)