FastQCFastQC Report
Mon 27 Feb 2023
SRR3127543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964090
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270730.9133663282828793No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC53360.18002152431268956No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG50450.17020400865020968No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG43740.14756636944222343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.085.140981
TAGGGCA19000.078.406284
CGTAGGG7050.077.991852
AAGGGAT62750.075.265774
AGGGATG50650.074.96825
ATAGCGG4900.074.8640751
TAGGGCG6150.074.1210864
GAGGGAT52450.073.29144
CGAGGGA19700.073.236013
GTAGGGC15300.073.103483
ACGGGTA3800.072.9648364
AGGGATC33000.072.912365
TAGGGAT30700.072.711054
CGAAGGG29250.071.818142
GTAGGGA28350.071.7773363
TAAGGGA32950.071.740613
AGGGATT47950.071.4470145
TAGCGGG13900.070.999782
ATAACGG2400.070.5451
AGTAGGG50250.070.328972
Produced by FastQC (version 0.11.5)