FastQCFastQC Report
Mon 27 Feb 2023
SRR3127542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546209
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368221.03834827558105No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC53020.14951177440472346No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG46270.1304773632913345No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG45740.12898280952983876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118200.084.099191
CGTAGGG7900.077.9303742
TAGGGCA23250.074.385354
ATAGCGG6550.072.542051
AAGGGAT87950.072.1908344
AGGGATG61300.071.989255
GAGGGAT69800.071.975524
ATAACGG4350.071.378021
ATAGGGA40800.071.300573
AGGGATC42900.070.110855
AGGGATT66900.069.826915
TAGGGCG7000.069.822894
CGAGGGA25150.069.7000963
GTAGGGA33200.069.644943
AGAGGGC60100.069.595013
AGTAAGG19100.069.458521
TAAGGGA42100.069.322163
GGTAAGG23050.069.189241
AAGAGGG131750.068.737482
CGAAGGG34650.068.629342
Produced by FastQC (version 0.11.5)