FastQCFastQC Report
Mon 27 Feb 2023
SRR3127540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472877
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127320.8644306347373202No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC45320.30769711252195536No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG38080.2585416161702572No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG37240.2528384922841486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50150.083.7667161
GGGCGAT4400.081.178096
ATAGCGG3000.079.972361
TAGCGGG5800.079.412842
CGTAGGG3450.079.013552
CGAAGGG12550.078.269982
TAGGGCG3350.077.160644
TAAGGGA18100.075.560073
GTAGGGC6100.075.504733
GCGGGAT7300.075.325314
AGGGATG31550.075.2314455
TAGGGCA11450.075.1144564
AGGGCAT17300.074.169265
AAGGGCA25000.074.06864
TACGGGT1600.073.4341353
GGCGATG3200.073.4341357
ATAGGGA21300.073.254863
AGGGCGA8100.073.1127245
CGGGAAT4450.072.8780065
TAGGGAT16550.072.41364
Produced by FastQC (version 0.11.5)