FastQCFastQC Report
Mon 27 Feb 2023
SRR3127539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789964
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178460.9970032916863133No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC41730.2331331803321184No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG37820.21128916559215719No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG37570.20989248945788852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.083.222761
AGGGATG37050.077.630165
AGTACGG2000.077.611741
TAGCGGG7250.077.139392
GTACGGG3900.077.122772
CGAAGGG15300.077.0991442
ATAGCGG4050.076.653571
TAAGGGA21850.076.356173
AGGGATC22600.075.693795
ATAGGGA24150.075.116863
GAGGGAT39300.074.979454
TAGGGCG3200.074.900974
AGTAAGG9400.074.559321
TAGGGCA14250.074.53514
AAGGGAT48300.073.754644
CGAGGGA12950.073.670493
AAGGGCA35350.073.386634
AGAGGGC30400.073.277733
ACGGGTA2450.072.892824
AGGGCGA10650.072.8117755
Produced by FastQC (version 0.11.5)