FastQCFastQC Report
Mon 27 Feb 2023
SRR3127538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356205
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126160.9302428467672661No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC39890.2941295748061687No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG34100.2514369140358574No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG33670.2482663019233818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46650.084.981181
ATAGCGG2600.081.586371
ACGGGTA900.078.52984
CGTAGGG3450.077.846932
AGGGATG27650.077.672725
TAGGGCA10650.076.53894
TCGAAGG2100.076.319951
AAGGGCG5050.075.575214
ATTGCGG1500.075.4220661
CGGGATT4550.075.3978355
AGAGGGC23650.075.109693
GGGCGAT4450.074.980066
GAATAGG9750.074.455111
CGAAGGG12250.074.23472
AAGGGAT36300.074.1165544
AGGGATT26200.074.0791855
TAGGGAT15800.073.6589664
ATAGGGA19750.073.4800343
AAGGGCA27450.073.466134
CGAGGGA10200.073.4484563
Produced by FastQC (version 0.11.5)