FastQCFastQC Report
Mon 27 Feb 2023
SRR3127537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986357
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90550.9180246097508307No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC26130.26491422476851684Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG25740.26096028111525543TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG25360.25710772063259046TruSeq Adapter, Index 22 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC12510.12683034641615562No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT9870.1000651893786935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1600.091.061764
CGTTTTT34350.087.467091
CGTAGGG2650.086.904952
CGGGTAT950.084.104585
GCGTAGG1950.081.981311
TAGGGTC3200.080.7805944
AGGGATG24450.080.351095
TACGGGT301.4560574E-678.33273
ACGGGAT5400.077.462334
ATAGCGG1700.077.442541
TAGGGCA7100.076.788114
AGGGAAT14900.076.334955
AGGGCAT9450.076.094625
TACGAGG1800.075.752331
AAGGGCA15600.075.319914
CGAAGGG8050.075.3161542
GGATGGC20100.073.886217
GAGGGAT16900.073.697634
AAGGGAT22500.073.528294
GGAATGC7450.073.176917
Produced by FastQC (version 0.11.5)