FastQCFastQC Report
Mon 27 Feb 2023
SRR3127536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180544
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123181.043417272037298No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG24480.20736202970833786TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG24440.2070232028624092TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC23670.20050078607828256Illumina Single End Adapter 2 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16360.13858017998482056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2550.090.313442
CGTTTTT43300.086.2685241
TAGGGTC2850.080.806764
CGGGTAT1050.080.5711755
AGGGATG28100.080.284445
TAGGGCA8650.079.8725744
ATAGCGG1900.079.234831
AGGGCAT12400.078.4594355
TAGGGCG2850.077.508534
GACCGAT800.076.377998
AAGGGCA17950.076.194754
AGGGCGT1550.075.806215
TCGTAGG750.075.2730941
CTAGCGG1000.075.2730941
AGGGAAT18500.075.199765
TAGCGGG5100.074.646822
TAGGGTA4100.074.5119554
GTAGGGT3900.073.512593
GGATGGC23300.073.424667
GGTAAGG5850.073.1821751
Produced by FastQC (version 0.11.5)