FastQCFastQC Report
Mon 27 Feb 2023
SRR3127535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888057
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89801.0111963533872261No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG23830.2683386314166771TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC23590.2656361021871344Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG22940.25831675219045624TruSeq Adapter, Index 22 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC11050.12442894994352839No Hit
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT9190.10348434841457249No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT9100.10247089995349397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2900.090.993552
GCGTAGG1500.084.8669051
CGTTTTT31700.084.331471
CGAAGGG6700.082.990392
TAGGGCG1350.080.281354
GGTAAGG4650.079.087431
AGGGATG22200.078.958915
TAGGGCA6400.078.7815254
AGGGCAT8750.075.19095
AGGGAAT13650.075.0531855
AAGGGAT21750.073.87814
AGGGCTC6400.073.428615
AAGGGCA14200.073.0053944
GGATGGC15800.072.8709267
ATAGCGG2200.072.8655241
TAGGGTC2150.072.326274
TAAGGGC9750.071.528273
TAAGGGA10050.071.2685853
AGGGCAA11650.070.99575
GTAGGGA7600.070.6824953