Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127535.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 888057 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 8980 | 1.0111963533872261 | No Hit |
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG | 2383 | 0.2683386314166771 | TruSeq Adapter, Index 22 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC | 2359 | 0.2656361021871344 | Illumina Single End Adapter 2 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG | 2294 | 0.25831675219045624 | TruSeq Adapter, Index 22 (95% over 23bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 1105 | 0.12442894994352839 | No Hit |
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT | 919 | 0.10348434841457249 | No Hit |
AGAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT | 910 | 0.10247089995349397 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTAGGG | 290 | 0.0 | 90.99355 | 2 |
GCGTAGG | 150 | 0.0 | 84.866905 | 1 |
CGTTTTT | 3170 | 0.0 | 84.33147 | 1 |
CGAAGGG | 670 | 0.0 | 82.99039 | 2 |
TAGGGCG | 135 | 0.0 | 80.28135 | 4 |
GGTAAGG | 465 | 0.0 | 79.08743 | 1 |
AGGGATG | 2220 | 0.0 | 78.95891 | 5 |
TAGGGCA | 640 | 0.0 | 78.781525 | 4 |
AGGGCAT | 875 | 0.0 | 75.1909 | 5 |
AGGGAAT | 1365 | 0.0 | 75.053185 | 5 |
AAGGGAT | 2175 | 0.0 | 73.8781 | 4 |
AGGGCTC | 640 | 0.0 | 73.42861 | 5 |
AAGGGCA | 1420 | 0.0 | 73.005394 | 4 |
GGATGGC | 1580 | 0.0 | 72.870926 | 7 |
ATAGCGG | 220 | 0.0 | 72.865524 | 1 |
TAGGGTC | 215 | 0.0 | 72.32627 | 4 |
TAAGGGC | 975 | 0.0 | 71.52827 | 3 |
TAAGGGA | 1005 | 0.0 | 71.268585 | 3 |
AGGGCAA | 1165 | 0.0 | 70.9957 | 5 |
GTAGGGA | 760 | 0.0 | 70.682495 | 3 |