FastQCFastQC Report
Mon 27 Feb 2023
SRR3127534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760458
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258540.9365837118333262No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC49490.17928184380997647No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG49280.17852110048405012No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG47800.1731596713299025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78900.082.6254041
GAATAGG14450.074.540971
CGTAGGG6950.074.395062
TAGGGCA15550.070.731654
AGAGGGC43500.070.344143
TAGAGGG53100.069.488242
GGTAAGG14450.068.6818541
AGGGTAC12900.068.135335
AAGGGCA47900.067.7083054
AGGGTAA12600.067.519395
AAGGGTA20500.067.180324
ATAGAGG19900.067.126291
AGTAAGG16200.067.069391
AAGAGGG97700.066.4408652
ATAGGGC18850.066.08013
AGTAGGG43900.065.95572
ATGAGGG42250.065.527682
AGGGCAA32950.065.047395
AGACGGG18950.064.987272
ATAAGGG37750.064.871952
Produced by FastQC (version 0.11.5)