FastQCFastQC Report
Mon 27 Feb 2023
SRR3127533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280991
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351701.0719322302316587No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG51580.1572085994749757No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC49260.15013756514418966No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG45040.1372755975252599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108500.082.558041
CGTAGGG7850.077.2334062
TAGGGCA23700.072.976764
AAGGGTA26200.072.471154
AGGGTAC16650.072.2621465
GAATAGG20800.072.152191
AGGGTAT21050.070.3304755
AGTAGGG55250.069.3282852
TAGAGGG65900.069.178552
AGGGCAT30850.069.0125665
AAGGGCA58250.068.98514
ATAGGGC24500.068.675533
AGAGGGC53100.068.594973
AATGCGG8250.067.860211
TAGGGTA10750.067.3282554
ATAAGGG48450.067.224042
GGATAGG16300.066.383841
AAGAGGG114500.066.167492
GAGGGTA20300.065.751764
AGGGTAA16100.065.6813055
Produced by FastQC (version 0.11.5)