FastQCFastQC Report
Mon 27 Feb 2023
SRR3127532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534279
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254661.0048617377960358No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG43730.17255400845763233No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC42700.1684897361340247No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG39910.1574806878011458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75500.082.571891
CGTAGGG6750.079.5515442
TAGGGCA15750.075.663694
GAATAGG14350.074.869041
AGTAAGG14100.073.522951
AGGGTAC12550.070.7734765
ATAGGGC17450.070.721823
TAGGGTA7600.070.6543354
AGGGCAT23200.069.2772455
AGGGTAT14950.069.156655
AAGGGCA44550.068.936214
AGGGTAA12450.068.6996465
AGTAGGG43050.068.602812
AAGGGTA19300.067.847694
GAGGGTA14850.067.5617144
ATGAGGG40900.067.4872742
GGTAAGG13000.067.420031
AGACGGG15050.067.289842
TAGAGGG48450.066.7898562
AAGAGGG89750.066.652562
Produced by FastQC (version 0.11.5)