FastQCFastQC Report
Mon 27 Feb 2023
SRR3127531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586284
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209070.8083798995005963No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG44050.17032158881236553No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC36970.14294640495784686No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG30340.11731116922967469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68050.083.757831
CGTAGGG7700.075.68852
TAGGGCA20700.072.198894
TAGAGGG47200.071.695092
GAATAGG18200.071.574661
ACGGGTA3300.071.208164
AGTAGGG43150.069.4926452
AGGGCAT29300.068.6514745
AGGGATG35150.068.590785
AAGGGAT44500.068.331044
ATAGCGG5050.067.9801941
TAGGGTA10450.067.91014
CGAAGGG25000.067.680172
AGGGTAC12150.067.304915
GTAGGGC16400.067.0598453
TAAGGGA23200.067.05493
AAGGGTA20800.066.88094
AGGGTAT16600.066.815575
AAGGGCA48050.066.803794
AGTAAGG16550.066.49191
Produced by FastQC (version 0.11.5)