FastQCFastQC Report
Mon 27 Feb 2023
SRR3127530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3079098
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272390.8846421906675266No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG37990.12338028864297271No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC36080.11717717331504227No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG30910.1003865417729478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97800.084.664471
CGTAGGG9650.077.436482
AGGGCAT35500.071.489335
ATAGCGG7150.069.747281
TAAGGGA31700.069.6813
ATAGGGC20550.069.524453
TAGGGCA23850.068.9692464
TAGAGGG58550.068.870972
ATAAGGG49950.068.779292
AGGGATG45150.068.700825
AGAGGGC43550.068.203193
CGAAGGG31450.068.14282
AAGGGCA59950.068.0465854
AAGGGTA25250.067.7510154
AGTAAGG19450.067.727621
AGTAGGG49900.067.7182
ATAGGGA29350.067.574073
GTAGGGC18700.067.354873
AAGAGGG112950.066.866022
TAAGGGC25950.066.828983
Produced by FastQC (version 0.11.5)