FastQCFastQC Report
Mon 27 Feb 2023
SRR3127529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364858
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202400.8558653415976774No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG37510.15861417472000433No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC30620.12947923300257352No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG27320.11552490678087225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68350.084.594681
CGTAGGG7050.073.493212
TAAGGGA22750.073.086473
ACGGGAT7100.071.6488344
ATAGCGG5050.070.918451
GCGATCG200.002164191870.492388
CGATCGG200.002164191870.492389
GGTAAGG16450.070.4703141
ACGGGTA3150.070.279884
AGGGCAT27550.070.2791445
AGAGGGC34000.070.23813
TAGGGCA17850.070.191924
TAGAGGG45100.069.870412
CGAAGGG24050.069.135852
AAGGGCA47250.068.2861254
AGGGATG31250.068.274215
AAGAGGG81950.068.167822
AGTAGGG38600.068.091132
AAGGGAT40550.067.3722154
TAGGGTA10150.067.2892464
Produced by FastQC (version 0.11.5)