FastQCFastQC Report
Mon 27 Feb 2023
SRR3127528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9670403
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3547953.668875019996581No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT240790.24899686186811448No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT216720.22410648242891223No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT191700.19823372407540824No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG119990.12407962729164441TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC111710.11551741949120424No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA106260.10988166677231548No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT102690.10618999022067642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT975250.090.257271
ATAGGGC45750.075.5533753
TAGTAGG32200.069.365531
GTTTTTT1294900.068.583492
AGTAGGG141500.067.927672
AAGGGGC351250.067.571434
AGATAGG48000.067.541151
AGTAAGG56550.067.162011
TAGGGCA32550.066.840534
ATAGGGA55900.066.720963
TAGAGGG170900.066.603142
GAATAGG55650.066.554691
ATAGAGG63300.066.029981
GGTAAGG56750.066.011941
GGATAGG53350.065.9792561
AATAGGG120300.065.7663652
TATAGGG69100.065.6309052
ATGTAGG27100.064.857591
AGGGGCT437600.064.629265
GATAGGG134100.063.8096432
Produced by FastQC (version 0.11.5)