Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127527.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6545819 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 214414 | 3.275587057937288 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT | 23374 | 0.35708289520379344 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT | 21741 | 0.33213567316786485 | No Hit |
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT | 18167 | 0.27753593553381173 | No Hit |
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG | 11265 | 0.17209458434460226 | TruSeq Adapter, Index 22 (95% over 21bp) |
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC | 10614 | 0.1621493047699608 | No Hit |
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC | 9035 | 0.1380270368001315 | No Hit |
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC | 7688 | 0.11744901592909916 | TruSeq Adapter, Index 20 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 59975 | 0.0 | 90.803764 | 1 |
ATAGGGC | 2900 | 0.0 | 72.440765 | 3 |
TAGTAGG | 2050 | 0.0 | 72.26404 | 1 |
AGTAGGG | 9550 | 0.0 | 69.24221 | 2 |
GTTTTTT | 79505 | 0.0 | 68.73703 | 2 |
GGATAGG | 3400 | 0.0 | 68.0537 | 1 |
AGATAGG | 3315 | 0.0 | 67.67066 | 1 |
AAGGGGC | 23500 | 0.0 | 67.33572 | 4 |
GAATAGG | 3480 | 0.0 | 67.029816 | 1 |
ATGTAGG | 1595 | 0.0 | 66.34182 | 1 |
ATAGGGA | 3175 | 0.0 | 66.3144 | 3 |
GATAGGG | 8735 | 0.0 | 66.17933 | 2 |
GGTAAGG | 3435 | 0.0 | 66.12809 | 1 |
TATAGGG | 4205 | 0.0 | 66.05438 | 2 |
AGTAAGG | 3700 | 0.0 | 65.33215 | 1 |
TAGAGGG | 11490 | 0.0 | 65.036514 | 2 |
ATAAGGG | 7610 | 0.0 | 64.35218 | 2 |
AATAGGG | 7675 | 0.0 | 64.17458 | 2 |
ATAGAGG | 4070 | 0.0 | 63.89933 | 1 |
GTATAGG | 1990 | 0.0 | 63.57184 | 1 |