FastQCFastQC Report
Mon 27 Feb 2023
SRR3127527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6545819
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2144143.275587057937288No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT233740.35708289520379344No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT217410.33213567316786485No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT181670.27753593553381173No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG112650.17209458434460226TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC106140.1621493047699608No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC90350.1380270368001315No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC76880.11744901592909916TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT599750.090.8037641
ATAGGGC29000.072.4407653
TAGTAGG20500.072.264041
AGTAGGG95500.069.242212
GTTTTTT795050.068.737032
GGATAGG34000.068.05371
AGATAGG33150.067.670661
AAGGGGC235000.067.335724
GAATAGG34800.067.0298161
ATGTAGG15950.066.341821
ATAGGGA31750.066.31443
GATAGGG87350.066.179332
GGTAAGG34350.066.128091
TATAGGG42050.066.054382
AGTAAGG37000.065.332151
TAGAGGG114900.065.0365142
ATAAGGG76100.064.352182
AATAGGG76750.064.174582
ATAGAGG40700.063.899331
GTATAGG19900.063.571841