FastQCFastQC Report
Mon 27 Feb 2023
SRR3127526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9640206
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3321533.44549691158052No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT248310.25757748330274266No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT222730.23104278062107803No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT201590.20911378864725505No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG121750.12629398168462375TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC116860.12122147597260888No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA106950.11094161265848468No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC99270.10297497792059629No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT98690.10237333102632869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT923750.090.3353041
ATAGGGC46450.071.632323
GGATAGG57300.069.39951
GGTAAGG55750.069.1342541
TAGTAGG32950.068.555151
AGTAGGG145450.068.276182
GTTTTTT1232100.068.1324842
ATAGGGA55800.067.462083
AAGGGGC347800.067.0754554
GAATAGG53950.066.81771
TAGGGCA29500.066.431724
ATAGAGG62950.066.385231
TAGAGGG171100.066.2576752
AGATAGG51200.065.810821
TATAGGG67850.065.7665942
AATAGGG120300.064.961252
GATAGGG138150.064.464122
AGTAAGG55450.064.4161151
ATGTAGG24400.063.839931
AGGGGCT436800.063.64055
Produced by FastQC (version 0.11.5)