FastQCFastQC Report
Mon 27 Feb 2023
SRR3127525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11530167
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130413.5822638128311586No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT235850.20455037641692442No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT218010.1890779205539694No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT206010.1786704390318024No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG143560.12450817061019151TruSeq Adapter, Index 22 (95% over 21bp)
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA128610.1115421832138251No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT122990.10666801270094353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1128300.090.1120451
ATAGGGC55500.073.1936953
TAGTAGG39100.069.921751
AGATAGG61350.068.5702741
GTTTTTT1505150.068.173242
GGTAAGG62700.067.994471
AGTAGGG172550.067.8249052
ATAGAGG79200.067.6723861
AAGGGGC426950.067.439874
TAGAGGG215500.067.049352
ATAGGGA63650.066.776383
GTATAGG37900.066.05191
ATGTAGG32050.065.1880341
GGATAGG63550.065.159851
GATAGGG162200.065.079592
GAATAGG65400.064.827611
AATAGGG145800.064.724562
AGTAAGG70050.064.554741
AGGGGCT527300.064.109735
AGGGACT136350.063.9993745
Produced by FastQC (version 0.11.5)