FastQCFastQC Report
Mon 27 Feb 2023
SRR3127524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12596328
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5014273.980739466295257No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT255580.20290040081522173No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT204300.16219012397898816No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT201500.1599672539489286No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG192900.1531398674280314No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA145000.11511291227094117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT141220.11211203773036078No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC130060.10325231289626628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1385500.090.6753541
ATAGGGC58300.071.4166263
TAGTAGG40250.069.553881
GTTTTTT1835200.068.943552
GGTAAGG72050.068.8803251
AGTAGGG182800.068.4343952
AGATAGG66900.067.701461
GAATAGG72550.067.561111
AAGGGGC452200.067.222414
ATAGGGA68100.067.156453
TAGGGCA39050.067.0609054
ATAGAGG79500.066.9903261
GATAGGG170950.066.5105742
TAGAGGG222900.065.73742
GGATAGG66950.065.539021
AATAGGG158650.065.2544562
AGGGGCT569850.064.9360355
TATAGGG87850.064.4980242
AGTAAGG74650.064.397821
AGGGACT153100.064.337915