FastQCFastQC Report
Mon 27 Feb 2023
SRR3127523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607139
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3061983.5574887311567758No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT248880.2891553163019675No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT214550.24926982124954647No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT203550.2364897325348179No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG186780.21700590637609082No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC130510.1516299434690203No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC99470.11556685676854991No Hit
CGTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC95590.11105897093099112No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA88430.10274029500394963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT883400.090.702331
ATAGGGC41000.073.0183
TAGTAGG26350.070.671831
GTTTTTT1164300.069.0539252
AGTAGGG125950.067.987582
ATAGAGG52950.067.940271
GGATAGG48250.067.833421
GAATAGG46250.067.513111
TATAGGG54900.067.029942
AAGGGGC303650.066.954544
GTATAGG26050.066.7922061
AGATAGG43150.066.5874251
GGTAAGG46100.066.50871
AGTAAGG49250.066.36061
AATAGGG99600.065.778332
ATAGGGA41350.064.8985443
GATAGGG117550.064.7696842
TGTAGGG55500.064.5269852
AGGGGCT379650.064.097775
TAGAGGG151500.063.5636372