FastQCFastQC Report
Mon 27 Feb 2023
SRR3127521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126607
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5832513.855795288394813No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT256290.16942993230405207No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG229560.15175908252260403No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT224060.14812310520131844No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT221220.1462456187299637No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA172770.114215963963366No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT169430.11200793409916712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1637200.090.1421
ATAGGGC69700.071.3709263
TAGTAGG54500.071.315021
ATAGAGG98500.068.646441
GTTTTTT2175450.068.499422
AGTAGGG230600.068.3713762
AAGGGGC547100.068.135814
GAATAGG88400.067.653581
GGTAAGG84400.066.5670551
AGATAGG77050.066.504751
ATAGGGA82300.066.100083
TAGAGGG280950.066.0766752
TATAGGG106950.065.817452
TAGGGCA49500.065.732384
AGGGGCT686850.065.526415
AATAGGG193650.065.221172
AGTAAGG88300.064.6394351
GGATAGG80750.064.3898241
GATAGGG203250.064.2227552
ATAAGGG186700.063.6193282
Produced by FastQC (version 0.11.5)