FastQCFastQC Report
Mon 27 Feb 2023
SRR3127520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179143
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301211.3822406331296295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86850.086.4593051
ATAGGGC9650.074.526573
ATAGCGG5050.071.692871
CGTAGGG8050.071.2378852
ACGGGTA2350.070.0079354
AGGGCAT18300.068.8383945
TAGGGCA10200.068.664644
AGTAGGG32700.068.56742
AATAGGG31500.067.001242
AGTAAGG12650.066.905011
TATAGGG14900.066.564582
GTACGGG5850.065.88782
TAGAGGG39300.065.783532
AGTACGG3650.065.698281
CGAAGGG22700.065.641792
AAGAGGG78200.065.33862
TAGCGGG13200.064.454272
TAGACGG2700.064.433931
AGGGACT30400.064.3230745
ATAGGGA18150.063.968683
Produced by FastQC (version 0.11.5)