FastQCFastQC Report
Mon 27 Feb 2023
SRR3127519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901647
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242841.2769983072568147No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG29720.1562855777123725No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC24430.12846758625549326Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG23650.12436587863047137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69250.086.584411
TAGGGCA9150.074.67224
TAGACGG2400.074.6353761
CGTAGGG6350.071.237642
ATAGGGC9150.070.037383
TATAGGG12400.068.401072
AGGGAAT19200.068.288685
AGTAGGG27750.068.261392
TAGAGGG31200.067.96262
GAATAGG9100.067.85821
GAGGGAT22050.067.7427444
AGGGCAT13300.067.13485
ATAGGGA14300.066.232643
ATGCGGG9700.066.0661852
CGAAGGG18050.066.047352
AATAGGG24400.066.046272
AGTAAGG10150.065.946951
GTACGGG4300.065.749862
ATAGAGG12600.065.469631
AGGGATG22500.065.1655045
Produced by FastQC (version 0.11.5)