FastQCFastQC Report
Mon 27 Feb 2023
SRR3127518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938814
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235761.2160011223356133No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG31010.15994314049723182No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC26320.13575309441751504Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG23570.12156916547951478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69900.087.0631261
ATAGGGC8200.076.798683
GTACGGG4150.070.251062
ACGGGTA2350.069.9943544
ACTAGGG8400.069.414742
AGTAGGG29650.068.988052
AGGGAAT18750.068.6771245
AGTACGG2400.068.63881
AGGGCAT14950.068.215235
CGTAGGG5900.067.744842
GTATAGG6650.067.2380141
TATAGGG13150.067.226642
AATAGGG25900.067.175552
TAGGGCA8150.066.8903054
AGGGTAC9200.066.407695
AGTAAGG10950.065.76431
ATAGGGA14000.065.4593
GAATAGG9000.065.370291
AGCAGGG37800.065.185172
GGATAGG11650.065.0448461
Produced by FastQC (version 0.11.5)