FastQCFastQC Report
Mon 27 Feb 2023
SRR3127517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851565
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT758452.6597675311627125No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT35980.12617632773582224No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT32870.11527003592763972No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT32820.11509469361561106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT229950.088.376551
GAATAGG16250.074.364461
ATAGGGC16900.072.319953
TAGAGGG51550.071.3098452
TAGGGCA13000.069.789974
ATAGGGA24250.069.591173
AGTAGGG43650.069.246552
CGTAGGG8650.067.930582
AGGGATG40100.067.75835
AGAGGGC42300.067.455873
ACGGGTA2800.067.155414
TAAGAGG22550.067.142111
GATAGGG44400.067.123982
ATAGAGG22650.066.638091
AGATAGG18250.066.472581
TATAGGG21350.065.612982
AAGAGGG103950.065.526252
TAGGGTA7300.065.039564
AGGGCAT26500.064.7479555
AATAGGG44600.064.6096042
Produced by FastQC (version 0.11.5)