FastQCFastQC Report
Mon 27 Feb 2023
SRR3127516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487588
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640152.573376298647525No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT46280.18604366961088412No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT44440.17864694635928458No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT42300.1700442356210112No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG33450.13446760476413297TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC30530.12272932656050761Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187700.089.477831
AGTAGGG37750.072.64162
TAGAGGG43300.069.42442
GAATAGG13800.069.3589861
AGGGATG32750.068.876815
TAGGGCA10950.068.847034
GAGGGAT27350.068.0485844
TAGGGTA6650.068.01884
ATAGGGC14450.067.822673
ATAGAGG19000.067.499581
TATAGGG17650.067.272132
AGATAGG13550.067.1589361
TAGGGCG4850.067.03274
GTAGGGA16500.066.535193
GGATAGG14450.066.239041
AGAGGGC32550.066.1522453
ATGAGGG37550.065.876232
AGGGCAT19750.065.434785
AAGGGGC81350.065.158994
ATAGGGA19200.064.786133
Produced by FastQC (version 0.11.5)