FastQCFastQC Report
Mon 27 Feb 2023
SRR3127515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537115
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623202.456333276181805No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT46170.18197834942444469No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT45210.17819452409528147No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT44680.1761055371948059No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG36020.14197227953797914TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC30120.11871751970249672Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT183400.088.044611
ATAGGGA18800.073.745833
TAGGGCG3500.072.510184
ATAGGGC14800.071.766213
GAATAGG13800.070.935821
AGGGATG35100.069.3578955
TAGGGCA10600.069.165894
GGTAAGG14100.068.091421
ATAGAGG19300.067.79051
TAGAGGG46100.067.3218462
AGTAGGG35100.067.118712
GATAGGG37700.066.730672
GAGGGAT27300.066.6226044
GTACGGG6150.066.5207442
AGGGTAC11300.066.1295
TAAGGGA16450.065.4248663
AGATAGG13800.065.138181
AGGGCAT20250.064.5198065
GTTTTTT253850.064.222872
TATAGGG17800.064.194712
Produced by FastQC (version 0.11.5)