FastQCFastQC Report
Mon 27 Feb 2023
SRR3127514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442614
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399861.1615011151409946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126300.083.535111
TAGGGCA18400.073.580224
AGGGATG55400.072.041775
CGTAGGG9450.070.140272
ATAGGGC19500.069.910683
AGTAGGG54100.068.558282
AGGGCAT35350.068.220425
TAGAGGG61750.067.982112
ATAGGGA28550.067.508413
GAATAGG21800.067.5052261
TAAGGGA28950.066.738033
AGTAAGG24350.066.4215551
GATAGGG51850.065.912192
TGTAGGG26050.065.686142
TATAGGG30000.065.655772
AATAGGG54400.065.414962
GACCGAT1150.065.408458
ATAGCGG9500.065.328081
TAGGGAT23500.065.013264
TAGCGGG21700.064.989272
Produced by FastQC (version 0.11.5)