FastQCFastQC Report
Mon 27 Feb 2023
SRR3127513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929281
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327841.1191824888086872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98150.083.572031
ATAGGGC16150.072.660643
ATAGGGA22200.072.176913
ATAGCGG6750.071.975571
AGGGATG42800.071.58865
TATAGGG23950.071.232042
CGTAGGG7550.071.1590652
GTAGGGT8700.068.794993
AATAGGG43050.068.528872
GAGGGAT34750.068.487134
TAAGGGA24550.067.763463
TAGGGCA15900.067.5787354
TAGACGG4750.067.525371
AGTAAGG19500.067.487181
AGTAGGG46950.067.252982
TAGAGGG49150.067.023332
GAATAGG19250.066.893451
AAGAGGG108200.065.5948942
AGAGGGA66400.065.225863
TGTAGGG20850.065.096672
Produced by FastQC (version 0.11.5)