FastQCFastQC Report
Mon 27 Feb 2023
SRR3127512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005755
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317761.0571719917291995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94850.083.0466461
ATAGGGC16800.072.7320563
ATAGGGA22000.072.416723
CGTAGGG8050.071.8471452
AGGGATG47000.070.694145
TAGGGCA16450.069.7084964
TATAGGG24500.069.285352
TAGAGGG51500.068.5698242
TAAGGGA23300.067.972893
AGGGCAT28950.067.5315255
TAGCGGG20000.067.476452
GAATAGG18450.067.330451
GTAGGGT9200.066.918363
TGTAGGG22450.066.8150942
TAGGGAT18650.066.2733154
GATAGGG41000.066.1747362
GGATAGG17750.065.744181
ATAGAGG19250.065.510061
AATAGGG43550.065.3232042
TAGGGTA8350.065.2879944
Produced by FastQC (version 0.11.5)