FastQCFastQC Report
Mon 27 Feb 2023
SRR3127511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534295
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87091.629998409118558No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35410.6627424924433132No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG18380.3440047164955689TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC15690.29365799792249603TruSeq Adapter, Index 27 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG12870.24087816655592884TruSeq Adapter, Index 27 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7810.14617393013223032No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC5820.10892858813951094No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT5800.10855426309435798TruSeq Adapter, Index 27 (100% over 22bp)
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG5690.10649547534601672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG156.8942504E-494.020321
CTAACGG201.8350225E-594.020311
CGTTTTT37350.088.482311
GTAGGGC1700.088.4731453
AGTACGG750.087.752291
ATAGCGG650.086.787981
TTACGGG1050.085.050072
AGGGATG13650.084.705745
CGGGTAT500.084.602455
TCCGCTA5600.083.107241
CGAGGGA2550.082.943573
GGATGGC12550.082.767337
CGTAGGG1600.082.252372
GATGGCA12100.081.57268
TAGCGGG1850.081.299642
AGGGAAT9050.080.499565
ATGGCAC12300.080.238719
GGGATGG13800.080.038546
CGCTACG5700.079.160193
TACGACC5750.078.471836
Produced by FastQC (version 0.11.5)