FastQCFastQC Report
Mon 27 Feb 2023
SRR3127510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491823
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69731.4177864800954816No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30820.6266482047403232No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG23310.4739509945651179TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC20610.41905319596684176TruSeq Adapter, Index 27 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG18480.37574493262820163TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC9800.19925867639374326No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT8540.17363970371454773TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7060.14354757707549262No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG6810.13846444757565224No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6810.13846444757565224No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6580.13378796843579904No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC5750.11691197849632896No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA5530.11243882453646943No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5480.11142219863650134No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT5400.10979559719655242TruSeq Adapter, Index 27 (100% over 22bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAC5400.10979559719655242No Hit
TAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT5390.1095922720165588No Hit
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT5330.10837232093659711No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5020.10206924035679503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG156.8333617E-494.229241
ATTGCGG156.8333617E-494.229241
ATCGGGT156.838901E-494.210033
CGAGGTA156.902841E-493.989738
CGAGGGA1650.088.500333
GTACGGG800.088.32192
CGTTTTT30600.088.2244341
CGATGGG1350.087.2315142
GATCCAA600.086.157268
GGATGGC10000.086.00067
GGGTACG550.085.4452066
AATGCGG1000.084.8063051
AGGGCAT4250.084.037885
AAGGGCA6900.083.969814
GTAGCGG452.7284841E-1183.7593151
GGGATCC3100.083.377996
AGGGATG11800.083.2366645
TAGCGGG1650.082.7906342
TCCGCTA5150.081.421371
CGCTACG5100.081.279243
Produced by FastQC (version 0.11.5)