FastQCFastQC Report
Mon 27 Feb 2023
SRR3127509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508084
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66951.3176954991694287No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30180.5939962683335827No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC26730.5260941104226861No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG23440.46134103809606286TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC21500.42315837538674705TruSeq Adapter, Index 27 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG20000.3936356980341833TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14360.28263043118854364No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG8040.1582415506097417No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT7990.15725746136465624TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7450.1466292975177333No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7300.14367702978247693No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG7200.14170885129230598No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6310.12419206272978484No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT5900.11612253092008408TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5870.11553207737303281No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAC5700.11218617393974224No Hit
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT5650.11120208469465678No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTC5630.11080844899662261No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG5540.10903708835546877TruSeq Adapter, Index 27 (100% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT5490.10805299911038334TruSeq Adapter, Index 27 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT5420.10667527416726368No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC5390.1060848206202124No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5360.10549436707316114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT301.3251338E-893.992423
CGTCGAT156.902189E-493.992429
GGCTCGT156.902189E-493.992427
TTACGGG1300.090.4307252
AGGGATG13400.088.731655
CGTTTTT36100.087.986371
GTAGGGC1800.086.159723
TAACGGG950.084.148182
GGATGGC11900.083.724357
AATCGGG452.7284841E-1183.598192
TAGCGGG2200.083.360692
GTGTAGG850.083.040731
GATGGCA11100.082.5609058
ATGGCAC11000.082.4569859
CTAACGG401.024091E-982.3487241
CGAGGGA2100.080.564933
CGGGCAA700.080.564935
CGGGAAT1400.080.564935
GGGATGG13200.080.107186
AATGCGG1000.079.99591
Produced by FastQC (version 0.11.5)