FastQCFastQC Report
Mon 27 Feb 2023
SRR3127508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448850
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310021.2659819915470527No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47060.19217183575964228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96400.086.136931
AGTAGGG36300.073.42082
TAGCGGG14950.072.000742
CGTAGGG7600.071.1257552
TAGGGCA12250.070.603214
ATGCGGG14750.070.4276052
AGTAAGG13800.070.188161
TAGAGGG47000.070.0072252
AGGGATG37000.069.745035
AGGGTAC12900.069.596335
AGGGAAT26850.067.399885
AGAGGGC36900.067.258973
ATAGCGG6650.067.1703641
ATAGGGC15200.066.487123
ATAAGGG36350.066.336972
TAAGGGA21100.065.717683
GAATAGG17050.065.633921
AAGAGGG87200.065.494142
GTAGGGT10700.065.455353
AAGGGTA16700.065.3001564
Produced by FastQC (version 0.11.5)