FastQCFastQC Report
Mon 27 Feb 2023
SRR3127507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132727
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250121.1727708234574796No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39460.18502133653299274No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG27360.12828646141770605TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC23900.11206310043432657TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG23340.10943735414799927TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77250.086.570021
CTACGAC8900.072.866595
CGTAGGG6600.072.843472
AGTAGGG32600.072.436012
GAGGGAT27250.069.533484
GTAGGGT9200.069.164033
AGAGGGC30350.068.798573
GTACGGG3850.068.5585562
CGAAGGG20450.068.453792
AGTAAGG13450.068.3757551
TAGAGGG39000.068.163032
ATGAGGG32100.068.13142
TAGGGTA8100.068.082464
GAATAGG12800.067.7951
AGGGATG31300.067.412645
TAGGGCA10450.067.205434
AAGGGCA32700.067.169564
GTAGGGA16300.067.086443
AGGGCAT19550.066.824765
ATGCGGG11600.066.637732
Produced by FastQC (version 0.11.5)