FastQCFastQC Report
Mon 27 Feb 2023
SRR3127506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174612
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240551.1061743428252948No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39800.1830211550382321No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG29910.13754177756767644TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG25140.11560683009198883TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC24580.11303165806130014TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75450.086.398431
CGTAGGG6650.077.795852
TAGGGTA8000.072.844624
AGTAGGG33750.072.462782
AGGGTAC10200.071.41635
GTAGGGA17250.071.107793
GTAGGGT9100.070.236573
AGGGATG31650.069.0470355
GAATAGG11700.068.790051
TAGCGGG10600.068.771942
TAGAGGG39850.068.6878742
AGGGAAT23200.068.469085
AGTAAGG12950.068.3287351
ATAAGGG30250.068.097972
ATAGCGG4700.068.096751
ATAGGGA20750.067.72033
GTAAGGG30400.067.1431352
AAGGGAC20800.067.105624
TAAGGGA16750.067.057733
TAGGGCA11450.066.903364
Produced by FastQC (version 0.11.5)