FastQCFastQC Report
Mon 27 Feb 2023
SRR3127505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333700
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647902.7762780134550287No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT30640.13129365385439432No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT29290.1255088486095042No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT27580.11818142863264343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT213250.089.480181
AGGGATG34500.071.394885
TAGGGCA11750.070.409244
ATAGGGA19350.068.505123
ATAGGGC13000.068.3397453
TATAGGG19200.068.3043752
CGTAGGG7100.068.190592
GTACGGG5000.067.687442
TAGAGGG43900.067.563232
AGGGCAT18350.066.858915
GGTAAGG14350.066.847031
ATGCGGG11850.066.243562
AGTAGGG35150.065.6601262
ATAGAGG16650.065.520551
AGAGGGC36250.065.354793
AGGGACT27450.065.243545
GATAGGG34600.064.9377752
GAATAGG13700.064.870171
AAGAGGG81700.064.783132
TAAGAGG18200.064.5910641
Produced by FastQC (version 0.11.5)