FastQCFastQC Report
Mon 27 Feb 2023
SRR3127504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017003
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550332.728454047911679No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT39050.19360407495675513No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT38430.19053020744143664No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT37220.18453120793573435No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG26170.12974695625142849No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC20660.10242919817174292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT175150.089.439071
AGGGATG29650.072.273475
ATAGGGC12000.071.8808443
CGTAGGG5600.071.5441742
TAGGGCA9250.071.339444
TAGAGGG35350.069.735782
ATAGGGA15350.067.555023
AGGGAAT20500.067.3957065
AGTAGGG29750.067.335692
AGTAAGG12300.066.708621
AGGGCAT16000.066.0847555
AGAGGGC31300.065.205913
GAATAGG11650.065.16851
TCGTAGG1450.065.0428851
TAGTAGG8150.064.803461
GTAGGGA12400.064.6205443
GGTAAGG12000.064.053681
GAGGGAT26000.063.8135154
TATAGGG17200.063.5774232
ACGGGAT4900.063.487944
Produced by FastQC (version 0.11.5)