FastQCFastQC Report
Mon 27 Feb 2023
SRR3127503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085116
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543432.606233897778349No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT41510.199077653233681No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT40610.19476134661093195No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT39960.19164401405005763No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG28580.1370667147535197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT176400.089.135161
TAGGGCA8650.076.063914
GTAGGGT6550.071.750573
ATAGGGC12100.071.465953
AGGGATG30100.070.104615
TAGGGTA6150.069.5397344
AGGGCAT16000.068.7325745
AGTAGGG31950.068.1536942
GAATAGG14650.067.782261
GATAGGG27550.067.771672
GGTAAGG12950.067.594961
AGAGGGC31400.067.202193
ACGGGTA2100.067.138054
ATAGGGA16000.066.3827443
AGTAAGG11450.066.174631
AATAGGG30700.065.873282
AAGAGGG67550.065.863532
TAGAGGG34100.065.649572
TGTAGGG12650.065.433692
TAAGGGA16750.064.813263
Produced by FastQC (version 0.11.5)