FastQCFastQC Report
Mon 27 Feb 2023
SRR3127502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790785
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464871.2263159213724861No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC69250.18267984071900673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144600.086.394261
TAGGGCA18650.073.348254
ATAGGGA33550.071.5975653
TAGGGTA12850.071.335734
CGTAGGG10750.070.4023742
AGGGATG59200.069.956795
GAATAGG24150.069.115081
AGATAGG22050.068.66081
ATAGGGC24100.068.65873
ACGGGTA4750.068.285914
ATGTAGG15900.067.421641
TGTAGGG33000.067.092952
TAGAGGG70550.067.030262
GTACGGG9500.066.305742
AAGAGGG147300.066.059842
ACTAGGG20200.065.857452
GGTAAGG20500.065.824811
AGGGAAT38600.065.763075
ATAGAGG31050.065.567391
AGAGGGC61950.065.484633
Produced by FastQC (version 0.11.5)