FastQCFastQC Report
Mon 27 Feb 2023
SRR3127501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242670
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379061.1689749496556852No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58510.18043772570135105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119100.086.263921
GAATAGG20650.074.926751
TAGGGCA15500.074.525074
ATAGGGA24800.073.764623
AGGGATG49550.072.8366855
ATAGGGC19300.071.333513
CGTAGGG8150.068.842622
TAGGGTA9400.068.716454
TAGAGGG59800.068.278622
GTACGGG8600.067.981582
AATAGGG48900.067.782022
ACGGGTA3200.067.775994
GGTAAGG18600.067.714461
AGGGAAT31200.067.627665
AGATAGG18700.067.60461
AAGAGGG117850.067.052122
AAGGGTA21700.066.920484
AGAGGGC50050.066.69563
AGTAAGG20800.066.675621
GAGGGAT44750.066.482044
Produced by FastQC (version 0.11.5)