FastQCFastQC Report
Mon 27 Feb 2023
SRR3127500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307730
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363121.0977921414383882No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60880.18405371659718295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112400.086.373111
CGGGTAT3350.074.350775
GACCGAT2350.073.99268
ATAGGGA26750.073.611323
AGGGATG52950.072.867125
AGATAGG19600.072.029571
GAATAGG20400.069.435561
TAGGGCA14650.068.327644
GGTAAGG17450.067.4202351
ATGTAGG12350.067.06431
ATAGGGC17750.066.984853
AAGGGTA19700.066.795354
AAGAGGG124850.066.434592
TGTAGGG28950.066.42922
AATAGGG52250.066.32322
TAGAGGG60500.066.294562
TAAGGGA30700.066.283273
AGAGGGC52250.066.1082153
AGGGAAT31050.065.687475
ATAGAGG25150.065.677221
Produced by FastQC (version 0.11.5)