FastQCFastQC Report
Mon 27 Feb 2023
SRR3127499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503034
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445821.2726682070456639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134100.086.284591
TAGGGCA19550.077.1786354
CGTAGGG9900.071.6936342
TAGGGTA13000.071.229794
ATAGGGA30350.070.777683
AGGGCAT30550.070.468185
TAGCGGG20900.070.16932
TAGAGGG60750.069.868322
AGGGATG54900.069.864515
AGTAGGG60900.069.773412
GTAGGGT12400.069.748513
ATAGGGC21250.068.792373
GAATAGG22450.068.6922761
ATAGCGG7650.068.220041
GAGGGTA16800.067.9885564
AGTAAGG21800.067.721041
TATAGGG28500.067.290562
TAAGGGA31350.067.0206763
AAGAGGG130450.066.948432
ATAGAGG25050.066.817881
Produced by FastQC (version 0.11.5)