FastQCFastQC Report
Mon 27 Feb 2023
SRR3127498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922660
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356701.2204635503274415No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC38710.13244783861277057No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG32570.11143957901363825TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG29620.10134603409223104TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104950.087.931821
TAGGGCA16100.076.983794
CGTAGGG7850.073.842242
AGGGATG41650.072.662885
GAATAGG17750.071.1990361
ATAGGGC18500.070.5630653
AGTAAGG16800.070.453431
TAGAGGG50000.069.465132
TAGGGTA9400.069.186384
AGTAGGG47600.068.116272
ATAGGGA23750.067.8628163
TAGCGGG19150.067.675792
GTACGGG7800.067.669482
TAAGGGA22400.066.6930543
AGAGGGC39550.066.490123
AGGGCAT25450.066.474715
AAGGGTA20300.066.3956154
ATAGCGG7600.066.390791
AAGAGGG103450.066.374072
AAGGGGC81600.066.0701454
Produced by FastQC (version 0.11.5)