FastQCFastQC Report
Mon 27 Feb 2023
SRR3127497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983490
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352281.1807648089988572No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC40310.13511022326201863No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG31640.1060502968000563TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG29950.10038578979651347TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107000.085.978751
TAGGGCA15050.080.5657654
AGGGATG45500.073.128935
TAGCGGG19050.072.569252
AGTAGGG50050.072.059562
ATAGGGA24700.071.9220663
TAGGGTA10700.070.714664
ATAGGGC17950.069.905953
GAATAGG18900.068.967751
GTAGGGT11150.068.2821963
TAAGGGA23000.068.043053
AGGGCAT28150.067.949045
CGTAGGG8800.067.3268362
TAGAGGG50850.067.319472
AGTAAGG19700.067.122511
ATAGAGG18700.066.685651
TATAGGG22000.066.2581562
GGTAAGG18450.065.803881
GTAGGGA23700.065.041853
AAGGGGC81850.064.882434
Produced by FastQC (version 0.11.5)