FastQCFastQC Report
Mon 27 Feb 2023
SRR3127496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159550
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187321.6154542710534256No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC82720.7133801905911776No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC52790.45526281747229524No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG43790.3776465007977233No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG35420.30546332629037126No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT20760.1790349704626795No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15640.13487991031003407No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT13010.1121986977706869No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCT12800.11038765038161356No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT12190.10512698891811478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74800.088.504531
CGCTACG15400.087.598073
TCCGCTA15650.086.825641
CTACGAC15550.086.450785
GGATGGC24000.083.8271267
ACGACCA15900.083.36897
GCTACGA16200.082.982084
TACGACC16150.082.947956
CGACCAA16150.081.496258
AGGGATG30600.081.104665
GCGGGAT5350.080.829034
GATGGCA22950.080.288898
CCGCTAC17250.078.4725952
AGGGAAT24250.077.725945
GGAATGC15050.077.458177
AACGGGA5600.077.220593
CGGGAAT5000.077.0862965
CGAGGGA8800.076.9153753
CGAAGGG8600.076.514582
AGCGGGA7700.076.304933