FastQCFastQC Report
Mon 27 Feb 2023
SRR3127495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069572
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148521.3885928203056923No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC71660.669987621216711No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC62070.580325588179197No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG52770.49337492006148254No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG46300.43288343374733074No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT24860.23242942036627734No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT17370.16240140916179557No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCT15590.14575923827474915No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCTTAACA14410.13472678791142625No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT14190.13266989038606097No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13490.12612521644171687No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT12570.11752364497200748No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT12460.11649519620932486No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC12420.11612121484107661No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT11970.11191392444828399No Hit
GAACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT11630.108735082818174No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT11360.10621070858249841No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT11130.10406031571507109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG12550.090.855753
TCCGCTA13150.089.627111
CTACGAC12900.088.5235755
CGTTTTT57450.088.379361
GCTACGA13150.086.710254
TACGACC13250.086.185216
CGACCAA13250.084.7665258
ACGACCA13600.082.930577
GGATGGC22250.081.949077
GCGGGAT5750.081.943214
AGGGATG27450.080.97695
CGAAGGG7350.080.772582
CCGCTAC14750.079.85992
TAGGGCA6900.079.211764
CGTAGGG1550.079.035542
TAAGCGG2150.078.938551
GATGGCA21100.078.8429958
CGGGATG5650.078.184695
CGAGGGA6800.077.6050343
AGGGTAC4450.077.091275