FastQCFastQC Report
Mon 27 Feb 2023
SRR3127494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103040
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145361.3178125906585436No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72810.6600848563968668No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC65050.5897338265158109No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG54530.49436103858427616No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG47650.4319879605454018No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCTTAACA38740.35121119814331303No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT26980.24459675079779516No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23620.21413548012764722No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT17820.16155352480417753No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCT15570.14115535248041777No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT15290.13861691325790543No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT13830.12538076588337685No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13780.12492747316507108No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT13040.11821874093414562No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC12550.11377647229474906No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT12220.11078474035393095No Hit
GAACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT12220.11078474035393095No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG11890.10779300841311285No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT11380.10316942268639397No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT11170.10126559326950972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.088.136941
TCCGCTA13800.087.6477661
CGCTACG13700.086.791263
GCTACGA13700.086.448214
AGTACGG1200.086.28361
CTACGAC13700.086.105165
GGATGGC21900.083.050637
AGGGATG26700.082.7299355
TACGACC14500.081.6786656
GCGGGAT5050.080.966234
CGACCAA14300.080.849098
AGCGGGA7200.079.6348953
ACGACCA14650.079.238357
CGGGATG6500.078.8114245
GGAATGC13200.078.685457
TAGGGCA6550.078.2098164
AGGGAAT24050.078.1665655
AAGGGCA15950.078.0838554
AGGGCAT9750.077.606375
CGGGAAT4000.077.546115
Produced by FastQC (version 0.11.5)