FastQCFastQC Report
Mon 27 Feb 2023
SRR3127493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183501
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403171.266435914422518No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38390.12059050711779264No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC36170.11361705242121803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126750.085.563191
ACGGGTA3400.080.1917344
TAGGGCA20200.074.702334
CGTAGGG9000.073.123882
AGTAGGG51700.070.7396242
TAGAGGG54950.068.951072
ATAGGGC20950.068.6611563
GAATAGG24200.068.183471
AAGGGTA22900.068.152714
TATAGGG26200.067.462162
TAAGGGA27500.067.0080643
TAGGGTA10050.066.888354
AATAGGG52850.066.79882
CGAAGGG32600.066.47482
AGGGATG44250.066.290585
AGGGCAT26900.066.231945
TGTAGGG25250.065.5322342
GTAGGGC18250.065.425123
ATAGGGA30300.065.315043
GTAGGGT11600.065.244153
Produced by FastQC (version 0.11.5)